Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (F1)

Name: Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (F1)
SKU: TAB-180CT
Rating: 5 (1 reviews)

CAT#: TAB-180CT

Recombinant monoclonal antibody to GM-CSF (Granulocyte-macrophage colony stimulating growth factor). This antibody intended for the prophylaxis and treatment of Rheumatoid arthirtis.

Published Data Gene Expression Tested Data

Neut

Figure 1 Neutralization by mAbs against GM-CSF of bioactivity of GM-CSF.

Washed TF-1 cells were incubated with titrations of the indicated mAbs and 200 pg/mL rhGM-CSF. After 4 d, metabolic activity of TF-1 cells was measured by the water-soluble tetrazolium assay.

Wang, Y., Thomson, C. A., Allan, L. L., Jackson, L. M., Olson, M., Hercus, T. R., ... & Waddell, T. K. (2013). Characterization of pathogenic human monoclonal autoantibodies against GM-CSF. Proceedings of the National Academy of Sciences, 110(19), 7832-7837.

Inhib

Figure 2 Neutralization by mAbs against GM-CSF of bioactivity of GM-CSF.

mAb F1 inhibits CD11b expression level on primary neutrophils stimulated by GM-CSF. Titrations of F1 mAb were added to heparinized blood with a high concentration of GM-CSF (10 ng/mL).

Figure 3 Neutralization of the bioactivity of GM-CSF by mAbs against GM-CSF.

After incubation with GM-CSF and titrations of mAb F1, PE-labeled anti-CD11b antibodies were added to the blood and held on ice. The cells were washed, and the neutrophils were gated on high side-scatter. Shown are histograms of fluorescence with different concentrations of mAb F1. Positive controls were GM-CSF (10 ng/mL) with no F1, and negative controls were neutrophils with no GM-CSF and F1 (300 ng/mL).

❮

❯

Subcellular Location Figure 1 IF staining of human cell line BJ

Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57404/1703_H9_1_selected.jpg

RNA Expression Figure 2 RNA cell line category: Cell line enriched (BJ hTERT+)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164400-CSF2

❮

❯

SDS-PAGE

Figure 1 Anti-Human GM-CSF Recombinant Antibody (F1) (TAB-180CT) in SDS-PAGE

SDS-PAGE analysis of TAB-180CT in non-reduced (lane 1) and reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

SEC-HPLC

Figure 2 Anti-Human GM-CSF Recombinant Antibody (F1) (TAB-180CT) in SEC-HPLC

The purity of this antibody is> 95% as determined by SEC-HPLC.

Figure 3 Anti-Human GM-CSF Recombinant Antibody (F1) in WB.

Western blot analysis of TAB-180CT was performed by loading Human GM-CSF Protein in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg, 0.3 μg, 0.6 μg) onto a 15% Tris-HCl polyacrylamide gel. Proteins were transferred to an NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-180CT (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Figure 4 Anti-Human GM-CSF Recombinant Antibody (F1) in DB.

Dot Blot analysis of TAB-180CT was performed by coating with Human GM-CSF Protein.
TAB-180CT incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000).

ELISA

Figure 5 Anti-Human GM-CSF Recombinant Antibody (F1) in ELISA.

ELISA analysis of TAB-180CT was performed by coating with Human GM-CSF Protein. Then blocked with BSA and incubated with TAB-180CT. The HRP-conjugated goat anti human IgG as a secondary antibody (1: 2000). Detection was performed using TMB
substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

❮

❯

Specifications

Host Species
Human

Type
Human antibody

Specificity
Human

Species Reactivity
Human

Clone
F1

Applications
Neut, Inhib, FC, ELISA

Related Disease
Rheumatoid arthirtis

Applications

Application Notes
The GM-CSF (GranuloCytotoxicitye-macrophage colony stimulating growth factor) antibody has been reported in applications of Neutralization, Inhibition, Flow Cytometry, Enzyme-linked Immunosorbent Assay.

Target

Alternative Names
GMCSF

Gene ID
1437

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for TAB-180CT. Click the button above to contact us or submit your feedback about this product.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-180CT, RRID: AB_3111854)

Copy citation

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-180CT.pdf

MSDS

MSDS - TAB-180CT.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "Clone F1"

CAT
Product Name

See other products for "CSF2"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
TAB-183CT	Mouse Anti-CSF2 Recombinant Antibody (TAB-183CT)	ELISA, FC, WB, Block	Mouse IgG
TAB-185CT	Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (4K21)	ELISA, Neut
TAB-183CT-S(P)	Mouse Anti-CSF2 Recombinant Antibody; scFv Fragment (TAB-183CT-S(P))	ELISA, FC, WB, Block	Mouse scFv
TAB-183CT-F(E)	Mouse Anti-CSF2 Recombinant Antibody; Fab Fragment (TAB-183CT-F(E))	ELISA, FC, WB, Block	Mouse Fab
TAB-185CT-F(E)	Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody Fab Fragment (4K21)	ELISA, Neut

CAT	Product Name	Application	Type
TAB-184CT	Human Anti-CSF2 Recombinant Antibody (TAB-184CT)	ELISA, FC, WB, Block	Chimeric (Mouse/Human) IgG1, κ
TAB-184CT-S(P)	Human Anti-CSF2 Recombinant Antibody; scFv Fragment (TAB-184CT-S(P))	ELISA, FC, WB, Block	Human scFv
TAB-184CT-F(E)	Human Anti-CSF2 Recombinant Antibody; Fab Fragment (TAB-184CT-F(E))	ELISA, FC, WB, Block	Chimeric (Mouse/Human) Fab

CAT	Product Name	Application	Type
TAB-186CT	Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (hGM4/1-34)	ELISA, Neut	Humanized antibody
TAB-186CT-S(P)	Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody scFv Fragment (hGM4/1-34)	ELISA, Neut	Humanized antibody
TAB-186CT-F(E)	Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody Fab Fragment (hGM4/1-34)	ELISA, Neut	Humanized antibody

CAT	Product Name	Application	Type
TAB-177CT-F(E)	Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody Fab Fragment (KB002)	ELISA, Neut	Human antibody
TAB-178CT-F(E)	Human Anti-CSF2 Recombinant Antibody; Fab Fragment (TAB-178CT-F(E))	ELISA, Neut, FC, WB	Human Fab
TAB-179CT-F(E)	Human Anti-CSF2 Recombinant Antibody; Fab Fragment (TAB-179CT-F(E))	ELISA, WB, Neut	Human Fab
TAB-180CT-F(E)	Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody Fab Fragment (F1)	ELISA, FC, Neut	Human antibody
TAB-181CT-F(E)	Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody Fab Fragment (C3)	ELISA, FC, Neut	Human antibody

CAT	Product Name	Application	Type
NEUT-511CQ	Recombinant Mouse Anti-CSF2 Antibody (CBL568)	Neut	IgG1
NEUT-513CQ	Rat Anti-CSF2 Recombinant Antibody (clone 23B6)	Neut	Rat IgG2a
NEUT-515CQ	Mouse Anti-CSF2 Recombinant Antibody (NEUT-515CQ)	IHC, Neut, WB	Mouse IgG1
NEUT-516CQ	Mouse Anti-CSF2 Recombinant Antibody (clone BVD2-21C11)	Neut, FC, ICC, IF, WB, IP, IHC-Fr	Mouse IgG2a, κ
NEUT-517CQ	Rat Anti-CSF2 Recombinant Antibody (clone BVD2-23B6)	ELISA, FC, IP, Neut	Rat IgG2a

CAT	Product Name	Application	Type
NEUT-512CQ	Rat Anti-CSF2 Recombinant Antibody (clone 22E9), Biotin	Block, ELISA, WB	Rat IgG2a
NEUT-514CQ	Rat Anti-CSF2 Recombinant Antibody (clone 31G6)	Block, ELISA, WB	Rat IgG1
NEUT-525CQ	Rat Anti-Csf2 Recombinant Antibody (clone CBL251)	Inhib	Rat IgG2a

CAT	Product Name	Application	Type
MOR-4580	Hi-Affi™ Rabbit Anti-CSF2 Recombinant Antibody (clone TH92DS)	WB	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0431-CN	Human Anti-CSF2 Recombinant Antibody (HPAB-0431-CN)	ELISA, FC, Neut	Human IgG1, κ
PABC-337	Human Anti-CSF2 Recombinant Antibody (clone F1)	ELISA, FC, Neut	Human IgG
HPAB-N0052-YC	Human Anti-CSF2 Recombinant Antibody (HPAB-N0052-YC)	ELISA, Block	Humanized IgG
VS3-WK1680	Recombinant Rabbit Anti-CSF2 Capture Antibody (clone R06-1A1)	ELISA	Rabbit IgG
VS3-WK1681	Recombinant Rabbit Anti-CSF2 Detection Antibody (clone R08-2Y7)	ELISA	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0431-CN-S(P)	Human Anti-CSF2 Recombinant Antibody; scFv Fragment (HPAB-0431-CN-S(P))	ELISA, FC, Neut	Human scFv
HPAB-1090-FY-F(E)	Human Anti-CSF2 Recombinant Antibody; Fab Fragment (HPAB-1090-FY-F(E))	ELISA	Human Fab
HPAB-1091-FY-F(E)	Human Anti-CSF2 Recombinant Antibody; Fab Fragment (HPAB-1091-FY-F(E))	ELISA	Human Fab
HPAB-1092-FY-F(E)	Human Anti-CSF2 Recombinant Antibody; Fab Fragment (HPAB-1092-FY-F(E))	ELISA	Human Fab
HPAB-1093-FY-F(E)	Human Anti-CSF2 Recombinant Antibody; Fab Fragment (HPAB-1093-FY-F(E))	ELISA	Human Fab

CAT	Product Name	Application	Type
HPAB-0431-CN-F(E)	Human Anti-CSF2 Recombinant Antibody; Fab Fragment (HPAB-0431-CN-F(E))	ELISA, FC, Neut	Human Fab
HPAB-0014-FY-F(E)	Human Anti-CSF2 Recombinant Antibody Fab Fragment (HPAB-0014-FY-F(E))	ELISA	Human Fab
HPAB-0136-FY-F(E)	Human Anti-CSF2 Recombinant Antibody (clone MSB-0010628); Fab Fragment	Inhib	Humanized Fab
PFBC-337	Human Anti-CSF2 Recombinant Antibody (clone F1); Fab Fragment	ELISA, FC, Neut	Human Fab
PABJ-0112-F(E)	Human Anti-CSF2 Recombinant Antibody Fab Fragment (PABJ-0112-F(E))	ELISA, Neut	Human Fab

CAT	Product Name	Application	Type
AFC-TAB-259	Afuco™ Anti-CSF2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-259)	IP, IF, FuncS, FC, Neut, ELISA	ADCC enhanced antibody
AFC-TAB-H44	Afuco™ Anti-CSF2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H44)	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS-0424-XY70	AbPlus™ Anti-CSF2 Magnetic Beads (BVD2-21C11)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0425-FY98	Human Anti-CSF2 (clone MSB-0010628) scFv-Fc Chimera	Inhib	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0525-XY1707	Anti-CSF2 Immunohistochemistry Kit	IHC

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 2 Anti-S. Aureus Recombinant Antibody (TAB-029) in HPLC

Anti-S. Aureus Recombinant Antibody (TAB-029) (CAT# TAB-029)
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC

Figure 2 Anti-Human CD2 Antibody (TAB-104) in WB

Anti-Human CD2 Recombinant Antibody (TAB-104) (CAT# TAB-104)
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC

Figure 2 Anti-Human CD18 Recombinant Antibody, hIgG1 (TAB-154-F(E)) in HPLC

Anti-Human CD18 Recombinant Antibody F(ab')₂ Fragment (TAB-154-F(E)) (CAT# TAB-154-F(E))
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

Figure 4 Anti-CD33 Recombinant Antibody (TAB-756) in SEC-HPLC

Anti-CD33 Recombinant Antibody (TAB-756) (CAT# TAB-756)
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC

Figure 1 Trastuzumab (TP-083CL) in SPR

Human Anti-ERBB2 Rcombinant Antibody (TP-083CL) (CAT# TP-083CL)
Application:

Figure 5 Mouse Anti-His Tag Recombinant Antibody Fab Fragment (PFBL-134) in HPLC

Mouse Anti-His Tag Recombinant Antibody (clone YC103); Fab Fragment (CAT# PFBL-134)
Application: WB, ELISA, FuncS

Figure 3 Human Anti-MS4A1 Recombinant Antibody (PABL-422) in Flow Cytometry

Human Anti-MS4A1 Recombinant Antibody (clone 2F2) (CAT# PABL-422)
Application: WB, ELISA, FuncS

Figure 2 Anti-Mouse F11 Recombinant Antibody (TAB-020WM) in HPLC

Mouse Anti-F11 Recombinant Antibody (TAB-020WM) (CAT# TAB-020WM)
Application: WB

Figure 5 Human Anti-Mouse Prnp Recombinant Antibody Fab Fragment (D18) (MRO-944LC-F(E)) in DB

Anti-Mouse Prnp Recombinant Antibody Fab Fragment (D18) (CAT# MRO-944LC-F(E))
Application: ELISA

Figure 2 Recombinant Mouse Anti-Streptococcus pneumoniae 23F Antibody (PABX-173) in HPLC

Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody (023.102) (CAT# PABX-173)
Application: Neut, ELISA, FuncS

Figure 4 Mouse Anti-TNFSF4 Recombinant Antibody (NEUT-2204CQ) in ELISA

Mouse Anti-TNFSF4 Recombinant Antibody (clone R4930) (CAT# NEUT-2204CQ)
Application: FC, Block

Figure 2 Human Anti-IL15 Recombinant Antibody (HPAB-0238-YC) in WB

Human Anti-IL15 Recombinant Antibody (HPAB-0238-YC) (CAT# HPAB-0238-YC)
Application: ELISA, FC, Inhib, FuncS

Figure 2 Human Anti-NOTCH4 Recombinant Antibody (HPAB-0068-CN) in HPLC

Human Anti-NOTCH4 Recombinant Antibody (HPAB-0068-CN) (CAT# HPAB-0068-CN)
Application: Neut, IF

Figure 1 Human IgG2, kappa Isotype Control Antibody (MOB-197CQ) in SDS-PAGE

Human IgG2, kappa Isotype Control Antibody (CAT# MOB-197CQ)
Application: FC, ELISA

Figure 2 CD19 Humanized Antibody (HPAB-0036LY) in HPLC

Recombinant Humanized Anti-CD19 Antibody (HPAB-0036LY) (CAT# HPAB-0036LY)
Application: WB, IHC

Figure 3 Recombinant Mouse Anti-CVA6 A-particles Antibody in HPLC analysis.

Recombinant Mouse Anti-CVA6 A-particles Antibody (1D5) (CAT# PABC-483)
Application: ELISA, Neut

Figure 3 Anti-LAM Recombinant Fab Fragment Antibody (PFBC-542) in WB

Recombinant Human Anti-LAM Antibody Fab Fragment (PFBC-542) (CAT# PFBC-542)
Application: ELISA, WB

Figure 2 Human Anti-HLA-B27 Recombinant Antibody (clone HD5) (HPAB-J0121-YC) in SDS-PAGE

Human Anti-HLA-B27 Recombinant Antibody (clone HD5) (CAT# HPAB-J0121-YC)
Application: ELISA, Block, WB, FC, IP

Figure 2 Human Anti-CLDN18 Antibody (ZG-0140U) in ELISA

Human Anti-CLDN18 Recombinant Antibody (clone 25E3) (CAT# ZG-0140U)
Application: ELISA, FC

Figure 1. Recombinant Rabbit Anti-VPS4A Antibody (clone R01-9H9) in WB.

Recombinant Rabbit Anti-VPS4A Antibody (clone R01-9H9) (CAT# VS3-FY1548)
Application: WB, IHC-Fr, IHC-P, ICC, IF

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.